If you’re diagnosed with a blood clot, your doctor may prescribe anticoagulant medications that thin your blood and prevent further clots. controlling high blood pressure, high cholesterol, and diabetes through diet and exercise.maintaining an appropriate weight for you.If you’re worried about blood clots, prevention is the best option. The likelihood of blood clots, DVT, and PE will be higher for someone with multiple risk factors for clots, such as: High cholesterol may also play a role in the development of DVT and PE, but more research is needed. High cholesterol, combined with other factors, can lead to blood clots in the arteries, known as PAD. throbbing or cramping pain that may be described as a severe muscle cramp. If a venous clot does cause symptoms, they may include: Your body will naturally break down and absorb the clot over weeks to months.Ĭatching a clot early is crucial for avoiding severe complications. Sometimes, a venous blood clot will dissolve on its own over time. DVTs are a type of venous clot.Īccording to the Centers for Disease Control and Prevention (CDC), about half of people with DVT have no symptoms. This type of clot can build up slowly over time and may not cause symptoms until it breaks off and becomes lodged in small blood vessels in other parts of the body. Symptoms include severe pain and coldness in the legs and feet.īlood clots that form in veins are known as venous clots. This type of clot requires emergency treatment, as it can lead to a heart attack or stroke.Īrterial blood clots that occur in the legs can lead to limb ischemia, which also requires emergency treatment. When a blood clot occurs in an artery, it’s called an arterial clot. This is called a pulmonary embolism (PE), which can be fatal.Įxactly how long a blood clot can go undetected will depend on its: If a portion of the DVT dislodges, it can move through your veins and eventually reach the arteries of your lungs.
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It’s a savvy choice that, combined with the heady chemistry between Ryan and Fanning, lets us share in some of the intimacy and immediacy they’re experiencing as they lose themselves in furious bursts of texts.Īs their conversations increasingly turn toward despair, The Girl From Plainville takes pains not to romanticize or condone Michelle and Conrad’s choices. The Girl From Plainville visually depicts these exchanges more or less like in-person conversations, so that when Conrad texts Michelle from his bedroom, she’ll speak her responses from the top of his bunk bed, clad in the baggy pink t-shirt he remembers from one of their few offline interactions. But they develop an intense bond online, trading thousands of messages into the wee hours of the night. With their Massachusetts hometowns separated by nearly an hour’s drive, the teens only meet face-to-face a handful of times after that initial vacation fling. In one of the show’s saddest, strangest interludes, a mourning Michelle imagines singing and dancing up and down her suburban street with Conrad like they’re Lea Michele and Cory Monteith’s characters from the latter. At the same time, we come to see how Conrad fits into the bigger picture of Michelle’s life, her visions for which seem to be informed by wildly romantic YA fare like The Fault in Our Stars and Glee. That structure allows Conrad to come to the fore as leading character in his own right - a bright but haunted young man with demons of his own, and not just a bloodless memory or symbol from someone else’s story. While detective Scott Gordon (Kelly AuCoin) and then assistant district attorney Katie Rayburn (Aya Cash) press on with their case against Michelle, culminating in a 2017 trial that unfolds over the last three episodes, the series simultaneously lays out a parallel chronological timeline of Michelle and Conrad’s lives, starting with their first chance encounter during separate family trips to Florida in 2012. In the immediate aftermath of Conrad’s death, Michelle ingratiates herself with his mother, Lynn (a ferocious Chloë Sevigny), who had no idea her son had a girlfriend at all at home, Michelle demands attention from her own family and friends, many of whom had never heard her so much as mention Conrad before his passing.īut in the seven hour-long episodes that follow, The Girl From Plainville shifts its focus from how the situation looked from the outside to how it felt from the inside for both Michelle and Conrad. The first episode, directed by Lisa Cholodenko ( The Kids Are All Right, Olive Kitteridge), is shot through with the uneasy feeling that something is off about Michelle, even if it’s not quite clear what. Initially, The Girl From Plainville seems to lean into the contemporaneous popular understanding of Michelle as a manipulative monster. She was indicted in 2015, at which point the story exploded all over the media. Shortly thereafter, local police discovered Carter had been egging him on via text messages and phone calls in the days and minutes leading up to his death. In 2014, Carter’s boyfriend, Conrad Roy III (played in the show by Colton Ryan), was found to have died by suicide. Cast: Elle Fanning, Colton Ryan, Chloë Sevigny, Norbert Leo Butz, Cara Buono, Kai LennoxĬreators Liz Hannah and Patrick Macmanus have based their miniseries on the 2017 Esquire piece of the same title by Jesse Barron, but odds are good much of the audience will be familiar with the basics whether or not they’ve come across that particular article. By ligating an adapter at the 3′ end of the cDNA fragment, eCLIP (similar to iCLIP) utilizes this termination to enrich for read pileups at these sites. Here, we describe a detailed protocol for seCLIP, a simplified, single-end version of the eCLIP methodology, as well as assorted notes for critical handling steps. The improved efficiency dramatically decreases experimental failure rates and PCR duplication, and enabled quantitative comparison with paired size-matched input to remove common CLIP artifacts. ), which incorporated high-efficiency enzymatic steps to achieve thousand-fold improved library efficiency. We recently described enhanced CLIP (eCLIP , which leads to high experimental failure rates and high wasted sequencing (often >90% of reads) due to the presence of substantial PCR However, widespread usage of these methods has been limited by the low efficiency of converting RNA molecules into adapter-ligated library Reads to enable identification of binding sites with single-nucleotide resolution. Step, iCLIP positions this crosslinking site at the start of the sequencing Reverse transcriptase enzymes can create deletions at these positions or, more often, terminate elongation due to the inability to read through this coupling, leading to a substantial fraction of cDNA fragments that terminate at the position of crosslinking. Treatment of UV-crosslinked protein-RNA complexes leaves at least one amino acid covalently crosslinked to its associated ribonucleotide. Various modifications of CLIP have since been described, including the use of photoactivatable nucleoside analogs (PNAs) to improve crosslinking efficiency (PAR-CLIP) and computational and experimental methods to identify binding with single-nucleotide resolution. In CLIP, RNA-protein interactions are stabilized via ultravioletĬrosslinking, a desired protein is immunoprecipitated using a factor-specific antibody, and associated RNA is isolated and converted into DNA library Building upon early RNA ImmunoPrecipitation (RIP) approaches that identified protein binding to entire transcripts, CrossLinking and ImmunoPrecipitation (CLIP)Įnabled high-resolution profiling of binding sites. Have made it possible to study RBPs and their RNA targets in an unbiased and transcriptome-wide manner. Recent advancements in next-generation sequencing Despite these known important roles, however, detailed studies to describe the targets of and regulatory mechanisms have largely focused on a small set of RBPs, with the majority of RBPs remaining poorly characterized. Recent studies have indicated that there are over 1500 RBPs, and this number is continuing to expand with additional studies. Of mRNA transcripts, targeting RNAs to specific organelles or subcellular localizations with the cell, controlling RNA stabilityĪnd turnover, and defining the timing and rate of translation Orchestrating these RNA processing and regulatory roles are RNA binding proteins (RBPs), which play a variety of roles including controlling alternative splicing These regulatory steps have been shown to play critical roles across a variety of developmental stages, and misregulation of RNA processing has been implicated in many human diseases, including cancer. RNA processing has been shown to play pivotal roles in shaping the cellular landscape through regulation of both protein-coding RNAs as well as regulation of processing and function of multiple classes of noncoding RNAs including long intergenic noncoding RNAs (lincRNAs) and small RNAs including microRNAs and piRNAs among others. These notes further refine the eCLIP methodology, simplifying robust RNA binding protein studies for all users. Further, we describe the observation of contaminating RNA present in standard nitrocellulose membrane suppliers, and present options with significantly reduced contamination for sensitive applications. In particular, we describe improvements to the adaptor strategy that enables single-end enhanced CLIP (seCLIP), which removes the requirement for paired-end sequencing of eCLIP libraries. Here we provide a step-by-step description of the eCLIP method, along with insights into optimal performance of critical steps in the protocol. The enhanced crosslinking and immunoprecipitation (eCLIP) methodology provides a framework for robust, reproducible identification of transcriptome-wide protein-RNA interactions, with dramatically improved efficiency over previous methods. Profiling of RNA binding protein targets in vivo provides critical insights into the mechanistic roles they play in regulating RNA processing.
Very nice original Model 404 Diamond T Truck with a factory St. Has some lower repairable quarter rust and minimal floor rust. Those are all there except 2 gone on the left rear fender. This was very well optioned with the chrome grill, bumper guard, stainless glass package, radio, stainless fender spheres and the stainless fender moulding on all 4 fenders. Here is a great rod project with great patina! Has the 216 six cylinder motor and 4 speed transmission. Shows 43,000 miles and is believed to be actual. The only rust I see is in the bolt in floor panel. This is the KS6 Series International with the overhead valve 6 motor, 4 speed transmission and 2 speed axle. This is a 1946 Chevrolet Pickup with more information coming soon! Good North Dakota Title and we can assist with delivery. Has a few bumps and bruises from 75 years of use. The truck will need a grill and they are available. Has the 6 cylinder motor and 4 speed transmission. Good North Dakota Title and we will assist with delivery. Otherwise it looks quite solid and not bad for dents. Has some rust showing inside above the crank out windshield and some in the bottom of the drivers door. Has a factory GM 12 foot stake bed with a hoist. This is a Model CC303 with the GMC 6 cylinder motor, 4 speed transmission and 2 speed rear axle. Good North Dakota Title and we will assist with transport. Will need the wood kit for the box floor. Has some lower body rust and some in the footwell area of the floor as well. The heads have been removed but are there. This 1941 has the flathead V-8 motor and the 4 speed transmission. Good Title and delivery can be arranged for you. Very popular body design! Don't miss this one. Has a decent body with the usual lower door rust and windshield header trim panel rust. Has a factory built 8 foot stake bed which could be converted to a pickup box if you wanted. Nice DecoArt Era Truck for restoration or rodding. Floors need some work but not bad either. The door bottom and cab corners are rusty. Good North Dakota Title and delivery is available.ĭecent truck for building. We also have available an IH pickup box from that era if you are going that route on a build. Has the IH flathead 6 motor and 4 speed transmission. If you are thinking of building a rod, check this one out! Great styling for a cool ride! Has some lower rust that will require some patch work and some dents from 80 years of use but all in all a good buildable unit. Find a door and here is a great start on a rat rod pickup. This cab has lower body rust in the body and floor structure. The trucks in this particular ad have been sold. Look at the ads below, give us a call or send us an email for information.Įmail at the individual ads below for cabovers for sale. We usually have cabovers in our inventory. This generally happens if your garlic is getting old and should be used up. I’ll be honest, I’m a researcher, not an experimenter. Traditional cooking wisdom tells us to remove this green part, because it will have a strong and bitter flavor. However, sometimes, it might be attached more firmly. And when this germ sprouts a little green stem, it means that the garlic has started to germinate and develop a sprout. This green part will usually seem like a separate sprout developing within the clove perhaps, you could simply pull this part out. The garlic bulb may also turn green in the centre, as it is trying to generate new leaves. The same could happen if a garlic plant is heavily fertilized and receives too many nutrients. When the garlic is taken out of the soil and exposed to the sun, it responds to the extra light and starts generating chlorophyll, thereby imparting a green colour to the inside of the garlic clove. As they are below the ground, the plant has no reason to produce chlorophyll there. The scape, if left on the plant, will form a flower and then seed (you can eat those tiny. These modified leaves tend to swell up in order to hold extra nutrients besides, they are white, because there isn’t any chlorophyll in them. The stalk is thicker than the leaves and is called the garlic scape. Garlic cloves are typically modified leaves, where the plant stores part of its energy beneath the ground. Let’s get into the details to learn more. Could be getting old, and hence is investing its energy into sprouting, which is generating new growth.Too much exposure to light has caused it to start producing chlorophyll, the green pigment.Garlic could be turning green for several reasons, including: The center focuses on three areas: performance, education and museum. The Center for Puppetry Arts, located in Atlanta, is the United States' largest organization dedicated to the art form of puppetry. And that’s exactly where Kermit, Miss Piggy and Jim Henson belong.Center for Puppetry Arts (the United States) Show map of the United Statesģ3☄7′33″N 84☂3′23″W / 33.792494°N 84.38985°W / 33.792494 -84.38985 With this opening, the Museum of Moving Image's new Henson exhibit opening in the winter and a new Muppets incarnation on TV, the Muppets are certainly in the spotlight once again. “We are working on a celebrity guest to cut the ribbon,” says Anthony - and it may just be a Muppet holding the scissors. While the Center isn’t ready to announce the exact plans for its November 14 grand opening ceremonies, Anthony says the Henson family will be in attendance and assures it will be a puppet-studded affair. The Center hired a full-time staff to help restore and conserve all of Henson's puppets to ensure they’ll be around for years to come. In fact, the conservation of the entire Henson collection was a huge undertaking. We were actually able to restore the foam to its original state,” says Fritz. Foam latex is a really brittle material and it doesn’t age well over time. “We’ve done an amazing amount of conservation work on these guys. When Jen and Aughra first came over to the Center, they were in need of a considerable amount of conservation and restoration. “They are really different from the look and feel of Henson’s more Muppet-y works,” says Fritz. Though the 1982 film was only a modest financial success at the time, it has since gained a cult following - and two of the film’s main characters, Jen and Aughra, can be found in the display. The museum will also show puppets from The Dark Crystal. The oldest piece on display - Omar - dates from Henson’s 1955 show Sam & Friends, which debuted when he was only 18 years old. But the museum’s staff is most excited to display Henson’s lesser-known puppets, like ones from his early years at the University of Maryland. Visitors will find about 75 Henson pieces on display and can also explore a reincarnation of the office where Henson illustrated, wrote and animated and an interactive creature shop.įrom Fozzie Bear to Fraggles, there’s no way a visitor won’t see one of their favorite characters. “Every time you come back, you’ll see something new,” Kelsey Fritz, the Center's exhibitions director, tells. While not every piece will be displayed at once, the museum plans to rotate the collection. After his death, his family donated over 500 Henson artifacts and the Center decided to expand its Atlanta facility to house them all. Henson was a strong supporter of the Center for Puppetry Arts during his lifetime. “He was not only a genius for puppetry, but for film and television innovation.” One of the collection’s primary objectives, he says, is to celebrate Henson’s talents across the spectrum - as a puppeteer, artist, and forward thinker. “Jim was a genius,” Vincent Anthony, the founder and executive director of the Center for Puppetry Arts, tells. On November 14, the highly anticipated $14 million, 7,500 square foot expansion of the Center for Puppetry Arts will have its grand opening - and the “world’s most comprehensive collection of Jim Henson puppets and artifacts” will highlight Henson’s life and artistic legacy. It’s time to meet the Muppets at Atlanta’s new Worlds of Puppetry Museum. 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